Silver nanoparticles (AgNPs) possess strong antimicrobial properties against various microbes, including those commonly found in biofilms [23]. Incorporating AgNPs into food packaging materials such as films or coatings can help prevent biofilm formation and reduce bacterial growth [24]. AgNPs disrupt bacterial adhesion and growth, inhibiting biofilm formation and maintaining the safety and quality of packaged food products [23]. The use of AgNPs against biofilm-forming microorganisms is outlined in Table 1.

A combination of AgNPs, gold nanoparticles (AuNPs), and chitosan has been shown to exhibit antibacterial characteristics, effectively inhibiting biofilm formation and reducing bacterial metabolic activity. Chitosan prevented biofilm development at concentrations ranging from 0.31 to 5 mg/ml, while nanoparticles combined with chitosan significantly reduced metabolic activity by at least 80%. The disruption of bacterial membranes by chitosan and AgNPs causes leakage of internal components from planktonic cells, further inhibiting microbial growth [23]. Additionally, combining zinc oxide nanoparticles (ZnONPs) with AgNPs on polyester surfaces has shown significant efficacy in preventing biofilm formation by harmful bacteria such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These nanoparticles also inhibit biofilms causing Listeria monocytogenes (L. monocytogenes). A combination of 850 ppm AgNPs and 400 ppm ZnONPs has demonstrated strong antibacterial effects, reducing E. coli CFU/cm² levels by 5.80 log and S. aureus by 4.11 log. In humid conditions, AgNPs at 500 ppm effectively prevent biofilm formation by L. monocytogenes [25].

Furthermore, functionalized stainless-steel surfaces with silver coupons exhibit lower counts of adherent S. aureus cells compared to E. coli. Cathodic sputtering of silver onto stainless steel lowers the microbial load on surfaces, with silver ions from the coating reducing both Gram-negative and Gram-positive bacteria. This highlights the potential use of silver-functionalized surfaces in the food industry to reduce microbial adhesion and biofilm formation [24]. Alumina discs coated with biogenic AgNPs show enhanced hydrophobicity and a significant reduction in microbial adhesion over 99.9% for both Gram-negative and Gram-positive bacteria, and over 90% for fungal isolates. These surfaces offer antibacterial properties, inhibit biofilm formation, and maintain biocompatibility with Caco-2 and HaCaT cell lines [26].

A study by Griffith et al. examined the antimicrobial effectiveness of silver zeolite coatings in minimizing contamination. Silver zeolite effectively prevents biofilm formation caused by common foodborne pathogens such as Listeria innocua (L. innocua) Seeliger and E. coli O157:H7. At a concentration of 0.3% w/v, silver zeolite inhibited L. innocua growth for up to 8 h. Its effectiveness against E. coli depended on dosage, showing reduced bacterial numbers after 4 h. This suggests silver zeolite’s potential as a coating material to enhance food safety and prevent microbial contamination on food-contact [27].

In a study conducted by [28], the antibacterial activity of two novel active films with silver, as IONPURE IPL, and ethyl lauroyl arginate (LAE®) respectively was assessed. One of these films was fabricated by incorporating silver in a low-density polyethylene (LDPE) matrix while the other was fabricated using an LAE® incorporated biofilm material INZEA F19. While silver incorporated LDPE films showed no antibacterial activity against E. coli and Aspergillus flavus, LAE® incorporated INZEA F19 reduced the growth of Salmonella enterica (S. enterica) but did not inhibit the growth of Aspergillus flavus. Interestingly, a 99.99% reduction in growth of Pseudomonas putida was observed when active polystyrene (PS) pads incorporated with LAE® were used. Notably, LAE® retained its antibacterial action against S. enterica even after thermal treatment at 180°C for 6 and 15 min (Fig. 1) [28].

The effects of AgNP-functionalized stainless-steel surfaces on biofilm formation and bacterial adhesion were thoroughly examined. Thin silver film coatings on polybutylene adipate-co-terephthalate (PBAT) and cellulose nanocrystal (CNC) nanocomposites showed improved crystallinity, ranging from 51% to 56%. These nanocomposites, created by solution casting with varying CNC concentrations (0.8, 1.5, and 2.3 wt%) and coated with silver thin films using magnetron sputtering, led to a significant 56% reduction in E. coli-induced biofilm, indicating potential use in food packaging [29]. Similarly, a poly (dimethyl siloxane) (PDMS) coating with AgNPs demonstrated remarkable antimicrobial activity, showing a 22-fold increase in effectiveness against E. coli and S. aureus compared to untreated PDMS. This was achieved using the solution blow spraying (SBS) technique to deposit AgNPs on partly crosslinked PDMS [30].

Additionally, the modification of a poly(butylene succinate-co-terephthalate) (PBST) matrix with Magnesium oxide (MgO)/AgNPs created PBST/MgO/Ag nanocomposite films. These films not only enhanced the mechanical, thermal, and barrier properties of the matrix but also exhibited excellent antibacterial activity against S. aureus, E. coli, and Salmonella paratyphi [31]. Furthermore, a study developed an antimicrobial polymer blend with immobilized fine silver nanophases (~25 nm), showing exceptional efficiency in inhibiting foodborne pathogens such as S. aureus and S. enterica. The biomaterial showed exceptional efficiency in inhibiting the mentioned pathogens, making it a promising biodegradable food packaging material [32].

Nanostructures made of silver, copper, zinc, titanium, and selenium have shown potential in the field of food safety and technology, despite potential hazards associated with some of them [34,35]. Table 2 presents the use of selenium nanoparticles (SeNPs) as antibiofilm food packaging materials. Selenium plays a role in thyroid hormone metabolism, cellular redox state regulation, and protection against reactive oxygen species. SeNPs were synthesized using the cell-free supernatant of Bacillus licheniformis, which was isolated from food wastes. Six foodborne pathogens, including Enterococcus faecalis, Bacillus cereus, E. coli O157:H7, S. aureus, Salmonella enteritidis, and S. Typhimurium, were found to be susceptible to the antibiofilm and antimicrobial properties of biosynthesized selenium nanoparticles with a diameter range of 10–50 nm [35]. The SeNPs showed a minimum inhibitory concentration (MIC90) of 25 mg/mL against all tested bacteria, effectively inhibiting their growth. Additionally, the SeNPs displayed antibiofilm activity at a concentration of 20 mg/mL against all examined bacteria, except B. cereus. Furthermore, the established biofilms were removed at concentrations of 75 mg/mL, and the SeNPs did not exhibit any toxicity to Artemia larvae [35]. Ullah et al. [21] conducted a study using SeNPs produced by Bacillus subtilis, and their antibacterial activity was assessed using the agar overlay technique. The results demonstrated that the SeNPs exhibited similar antibacterial effects against Pseudomonas aeruginosa, Salmonella typhi, and S. aureus at concentrations of 150 and 200 μg/mL. Notably, the SeNPs caused breakdown of the bacterial cell membrane in both S. aureus and P. aeruginosa. Significant anti-biofilm activity was observed at 700 μg/mL concentration against pathogenic strains capable of forming biofilms by SeNPs. The SeNPs dispersed biofilms of P. aeruginosa (85.7%), S. typhi (89.6%), and S. aureus (78.3%) within a time span of 15 min [21].

Metal oxide nanoparticles have been extensively studied for their potential in food packaging, particularly for their ability to combat microbial biofilms. Nanoparticles such as silver oxide, titanium dioxide (TiO₂), and zinc oxide (ZnO) offer strong antibacterial properties that help prevent microbial contamination [36]. When integrated into packaging, these nanoparticles release metal ions or generate reactive oxygen species (ROS) upon exposure to moisture or light, effectively inhibiting bacteria, fungi, and other microorganisms. Silver oxide nanoparticles, for instance, release silver ions (Ag⁺) that interact with microbial cells, disrupting their functions and preventing growth. Similarly, TiO₂ nanoparticles generate ROS, such as hydroxyl radicals, which damage microbial cell membranes [20,36].

Another approach to food packaging involves using metal oxide coatings to enhance antibacterial properties. A notable method includes the use of a nano-CuO coating on copper foil (Cu(CuO)), which produces reactive chlorine species (RCS) in situ, suppressing biofilms and killing bacteria on contact. This approach successfully treats bacteria like S. aureus and E. coli by employing heterogeneous Fenton chemistry to generate ROS, damaging bacterial cells. The concentration of H₂O₂ and Cl^– in the environment also influences bacterial growth. Furthermore, the NO₂-Cu-Fenton system has shown enhanced performance in eliminating biofilms and killing bacteria, proving particularly effective against E. coli and S. aureus in food and water [37]. Moreover, a blend film consisting of pullulan/collagen/ZnO nanoparticles has been found to be effective against A. niger and has shown potential as an edible food packaging material due to its water solubility [38]. Another antibacterial composite such as PAL@ZnO, created by incorporating natural palygorskite (PAL) nanorods into a chitosan/gelatin (CS/GL) matrix. This material enhances mechanical strength, water resistance, and thermal stability, while effectively combating E. coli and S. aureus [39]. Furthermore, anthocyanins extracted from purple potatoes or roselle were used to modify a chitosan/polyvinyl alcohol/nano-ZnO (CPZ) based film, which displayed strong antibacterial activity against S. aureus and E. coli, while also being capable of monitoring the spoilage status of packaged shrimp through changes in pH caused by microbial spoilage [40]. Additionally, antimicrobial nanocomposites containing ZnO-NPs with gelatin and tragacanth significantly inhibited bacteria such as S. aureus and E. coli, while enhancing the mechanical properties of the packaging [41]. The zinc sulfide nanoparticles (ZnS NPs) also possess antibiofilm activity and, like ZnO NPs, have potential applications in the food industry. Both ZnO and ZnS NPs were equally effective in killing bacteria, with S. aureus being more susceptible compared to P. aeruginosa and Klebsiella oxytoca [42].

Nanocomposite films composed of bovine gelatin and magnetic iron oxide (MIO) nanoparticles (5–20% w/w) have significantly improved mechanical and barrier properties, with optimal performance observed at a 10% concentration. Notably, the 20% MIO nanocomposites exhibited the highest antimicrobial activity against E. coli (7.1 mm) and S. aureus (8.22 mm). These gelatin/MIO films were further evaluated for their potential to extend the shelf life of grapes, underscoring their suitability as biodegradable packaging materials [43]. Liu et al. reported that soluble soybean polysaccharide composite films incorporating 5 wt% nano zinc oxide and 10 wt% tea tree essential oil exhibited enhanced water vapor barrier properties, thermal stability, and UV protection. Although there was no change in tensile strength and elongation, light transmittance at 600 nm decreased from 85.5% to 10.1%. These films demonstrated a DPPH radical scavenging activity of 67.7% and exhibited strong antibacterial effects against E. coli and S. aureus, indicating their promising potential for active packaging applications [44]. Additionally, an active packaging biofilm developed from biodegradable poly(butylene adipate-co-terephthalate) (PBAT), combined with 2% copper oxide nanoparticles (CuO-NPs) and varying amounts of enzymatically prepared nanocellulose fibers, showed enhanced thermal stability, improved mechanical strength, and robust antimicrobial activity against both Gram-positive and Gram-negative bacteria, as well as unicellular and filamentous fungi [45].

Purohit et al. examined the antimicrobial effects of nanoceria-infused chitosan-based biopolymer films for food packaging, finding significant antimicrobial activity against E. coli and L. monocytogenes, particularly under visible light. This antimicrobial effect is attributed to nanoceria’s ability to alternate between oxidation states (Ce³⁺ and Ce⁴⁺), which enhances its photocatalytic properties [46]. In a related study, Osman et al. explored hydroxypropyl-methyl cellulose (HPMC) films that incorporated Al₂O₃ and SiO₂ nanoparticles (NPs) to target foodborne pathogens such as Bacillus cereus, S. aureus, and S. Typhimurium on chicken fillets. The nanoparticles, approximately 80 nm in size and applied at a concentration of 80 ppm, exhibited substantial antimicrobial efficacy, reducing microbial loads by 2–3 log₁₀ CFU/cm² over 15 days of refrigerated storage [47]. Additionally, Momtaz et al. developed gelatin/chitosan (GEL/CS) nanocomposite films enhanced with nickel oxide nanoparticles (NiO NPs) for active food packaging. The inclusion of optimized concentrations of NiO NPs significantly improved the films’ mechanical, barrier, and thermal properties, with tensile strength increasing from 23.83 MPa (without NPs) to 30.13 MPa with the addition of 1% NiO NPs. These films demonstrated strong antibacterial activity against the Gram-positive bacterium S. aureus compared to the Gram-negative bacterium E. coli. Toxicity assessments further revealed that the films were non-toxic to fibroblast cells, achieving a survival rate exceeding 88% [48].

The development of new materials and coatings continues to push the boundaries of food packaging technology. For example, a photocatalyst called graphitic carbon nitride (g-C₃N₄) has been studied for its potential to prevent and remove biofilms. Unlike TiO₂, which requires ultraviolet light, g-C₃N₄ harnesses visible light. When exposed to white LEDs, it effectively disrupts biofilm formation, progressing from the surface to the core, facilitated by ROS diffusion [20].

Another study explored silver-graphene-TiO₂ nanocomposites for controlling the growth of Campylobacter jejuni. These nanocomposites induced leakage of proteins and DNA from bacterial cells, reduced bacterial motility and hydrophobicity, and significantly inhibited biofilm formation. Importantly, these synthesized nanoparticles were confirmed as non-cytotoxic to human cells [49]. According to the research findings, Similarly, nanocomposites combining silver, ZnO, reduced graphene oxide (rGO), and polyethylene glycol (PEG) showed robust anti-biofilm activity. the biofilm formation of Saureus and P. aeruginosa bacteria was robustly inhibited due to the pronounced anti-biofilm effects of AZGP nanocomposites. The AZGP nanocomposite, containing 0.05 M silver nitrate and a dosage of 31.25 μg/mL, inhibited around 95% of biofilm formation while maintaining antimicrobial activity for up to 90 days (Fig. 2) [50].

Silica nanoparticles (SNPs), a type of metal oxide nanoparticle, are effective in mitigating biofilms due to their high surface-to-volume ratio, which enhances interactions with microbial cells and biofilm matrices. In food packaging, SNPs can prevent microbial attachment and growth, disrupt biofilm structures, and exhibit antimicrobial activity by damaging microbial cell membranes, inhibiting biofilm formation [34].

One promising application involves SNPs encapsulating basil oil, incorporated into chitosan films. These basil oil@SiNPs films demonstrated antimicrobial inhibition against both Gram-positive and Gram-negative bacteria, as well as pathogenic yeast. While they increased the water contact angle and activation energy, they decreased water vapor permeability but increased oxygen permeability [51]. SNPs are also utilized as delivery platforms for biocidal agents in anti-biofilm research. When grafted onto polyethylene, polystyrene, and polyvinyl chloride surfaces, SNPs increased surface nanoroughness while maintaining mechanical properties. These grafted surfaces significantly reduced the adhesion of Gram-positive B. licheniformis or Gram-negative bacteria P. aeruginosa. This method offers a durable anti-biofouling solution for biomedical devices, food, water treatment systems, and industrial applications (Fig. 3) [34].

Moreover, a study demonstrated that stainless-steel surfaces coated with silica nanoparticles (SNPs) modified with polyethyleneimine (PEI) and polytetrafluoroethylene (PTFE) nanoparticles in an electroless nickel coating exhibited antimicrobial and nonfouling properties. These coatings reduced L. monocytogenes populations by over 5 log and decreased biofilm formation by more than 2 log. The coatings also inhibited exopolysaccharide production, lowering the risk of cross-contamination, nosocomial infections, and foodborne diseases (Fig. 4) [52]. Some recent studies outlined the application of various metal oxides conjugated films to mitigate microbial biofilm, as presented in Table 3.

Food packaging materials must meet specific criteria, with antimicrobial and antibiofilm packaging gaining importance. Eco-friendly, bio-derived polymers are increasingly replacing petroleum-based products. These biobased polymers include natural polymers (cellulose, chitin), synthetic polymers (polyhydroxy alkanoates [PHA], poly(glycolic acid) [PGA]), and biobased nanocomposites, which combine a biopolymer matrix with nanoparticles for enhanced antimicrobial properties [54,55]. Nanoparticles made from biopolymers and biosurfactants have also gained interest as they can be produced without harmful chemicals [9]. These materials are used in films, coatings, and emulsions, serving as active components to maintain food quality and safety [56,57]. Exopolysaccharides (EPS), polyhydroxy alkanoates (PHA), polylactic acid (PLA), starch, lignin, and protein-based polymers like collagen, gelatin, and gluten are currently under scrutiny for their potential as food packaging agents [58].

The effectiveness of various natural antimicrobial chemicals, such as citric acid, curcumin, calcium lactate, erythorbic acid, hop extract, garlic extract, and nisin, has been evaluated as food additives and in packaging materials. These agents were tested for their antibacterial and anti-biofilm properties against bacteria such as L. monocytogenes, S. aureus, and E. coli. It was found that garlic extract, erythorbic acid, and citric acid were the most effective in inhibiting bacteria. Moreover, combining two additives resulted in better efficacy, and mixtures of all these showed higher efficiency in inhibiting bacteria [59]. PEG-decorated GO Nanosheets have been demonstrated to enhance the efficiency of chitosan biopolymer, providing better thermal and mechanical stability as well as antibacterial properties [60]. Chitosan nanoparticles (CNPs) have also shown promise in preventing biofilm formation. CNPs at a concentration of 1500 μg/mL effectively suppressed biofilm formation by P. aeruginosa, S. aureus, and C. albicans by 91.83%, 55.47%, and 66.4%, respectively [61].

A recent study demonstrated the effectiveness of poly-L-aspartic acid nanoparticles with essential oil in combating S. aureus biofilm contamination on food-contact surfaces. This approach shows promise for applications in the food industry. The study confirmed the effectiveness of PASP in damaging S. aureus biofilms by impacting metabolic activity, bacterial adhesion, and extracellular polymeric substances. PASP was particularly effective in reducing the production of extracellular DNA (eDNA) by 49.4%. Additionally, nanoparticles containing essential oil (EO@PASP/HACCNPs) exhibited enhanced permeation and dispersion effects on biofilms, resulting in long-lasting anti-biofilm activity. When tested on a 72-h-old biofilm, EO@PASP/HACCNPs reduced the population of S. aureus by 0.63 log CFU/mL compared to the use of EO alone [62].

The use of nanocomposites in food packaging can help prevent the formation of biofilms on food. These nanocomposite biofilms are also useful in managing plastic waste and combating infectious diseases. For instance, Reference [22] demonstrated that electrospun fiber-based biofilms can act as a barrier against infectious microorganisms. By incorporating polylactic acid microfibers with active agents, nanocomposite biofilms with antimicrobial properties can be developed. The study revealed that acetylated cellulose nanofibers, with diameters of about 83 nm, and microfibrillar structures with diameters ranging from 1.7 to 2.4 µm, exhibited favorable characteristics for this purpose. Moreover, these nanocomposite biofilms demonstrated antibacterial activity against S. typhimurium and S. aureus [22]. In another study by Sun et al. it was found that starch-lignin nanoparticles composite films exhibited impressive tensile strength and elastic modulus, surpassing pure starch film. Additionally, lignin nanoparticles improved the thermal stability of the composite films and enhanced UV protection, reduced oxygen permeation, and scavenged DPPH free radicals, thus delaying the oxidation of soybean oil. Furthermore, bionanocomposites can effectively trap antimicrobial bioactive compounds [63]. Bionanocomposites are capable of trapping antimicrobial bioactive compounds which is a significant advantage. In a study by Cacciatore et al., it was demonstrated that carvacrol encapsulated into nanoliposomes and polymeric Eudragit^® nanocapsules significantly reduced the presence of bacteria such as S. aureus, L. monocytogenes, E. coli, and Salmonella spp. on stainless steel surfaces. The encapsulated carvacrol displayed regulated release and a subdued scent, making it a desirable option for food applications [64]. The application of electrospun biodegradable polymers in biomedical and food industries was explored by Rumbo et al. as well. Their study revealed that electrospun polycaprolactone (PCL) microfibrous mesh could become colonized by pathogenic bacteria. However, it was suggested that functionalizing PCL meshes with antimicrobial compounds can make them effective food packaging materials, preventing bacterial adherence and biofilm development (Fig. 5) [65].

The research conducted by [66] revealed that composite films made of ethyl cellulose and polydimethylsiloxane, and containing small amounts of clove essential oil, exhibited antibacterial properties against E. coli, S. aureus, and P. aeruginosa. The clove essential oil was found to enhance the hydrophobicity of the composite material, which comprised 20 wt% PDMS and 80 wt% ethyl cellulose. According to the study, the presence of clove essential oil on the composites seemed to inhibit the growth of biofilms [66]. Additionally, Reference [9] developed antibacterial nanoparticles by combining chitosan with the biosurfactant rhamnolipid. The addition of rhamnolipid reduced the size and polydispersity index of chitosan nanoparticles, leading to a higher positive surface charge and increased stability of chitosan/rhamnolipid nanoparticles (C/RL-NPs). These nanoparticles demonstrated antimicrobial activity against both planktonic bacteria and biofilms of Staphylococcus strains. Furthermore, moderate concentrations of NPs effectively eliminated both S. aureus and Staphylococcus epidermidis biofilms (Fig. 6) [9].

Antimicrobial packaging incorporates antimicrobial compounds into the packaging material to inhibit the growth of microorganisms [67]. This type of packaging not only helps prevent foodborne epidemics but also extends the shelf life and maintains the quality of food products. Starch-based materials, like chitosan, are commonly used as antibacterial agents against various microbes, and chitosan has been effectively applied as an edible coating with strong antibacterial properties [68]. Bio-nanocomposite materials, including chitosan and starch, play a critical role in food packaging due to their superior barrier qualities, which enhance food stability [17]. Starch is the most widely used biopolymer for food packaging. Various antimicrobial additives, such as tea tree essential oil, cinnamon essential oil, cassava starch, cellulose nanofibrils, neem, oregano, lemongrass essential oil, rosemary extract, and pomegranate peel powder, are being evaluated in combination with starch-based biopolymers as antimicrobial additives [69,70]. Furthermore, a modified antimicrobial peptide (1018K6) was bioconjugated to polyethylene terephthalate, demonstrating antibacterial and anti-biofilm capabilities against foodborne pathogens such as L. monocytogenes [71].

Microbial biofilm is a major concern in the food industry as it can be formed by several pathogens such as E. coli, S. enterica, Yersinia enterocolitica, C. jejuni, S. aureus, L. monocytogenes, and E. coli O157:H7 [72] These biofilms provide various advantages to the microorganisms, including forming a physical barrier against desiccation, mechanical resistance against liquid streams in food industries, and resistance to antimicrobial agents. Several methods, such as ultrasound, ultraviolet rays, magnetic fields, as well as the application of different chemicals and inhibitors, can disrupt biofilm formation and growth [73]. Studies have shown that incorporating phytochemicals like eugenol and thymol into packaging materials made of poly(lactic acid), poly(butylene succinate), and PBAT improves their antimicrobial properties against food-related pathogens like B. cereus and S. aureus [74]. Additionally, grafting CNPs with eugenol and carvacrol enhances their antimicrobial properties against E. coli and S. aureus [75]. An edible coating of nano-emulsion using carboxymethyl cellulose (CMC) and cardamom essential oil (CEO) was found to be effective against E. coli and L. monocytogenes. This coating material was also found to reduce the loss of fruit (tomato) weight significantly by maintaining firmness and reducing oxidative stress [76]. Furthermore, the use of food additives to inhibit biofilm formation on food can be a promising solution. Sodium citrate and cinnamic aldehyde have been reported to prevent the growth of biofilms of S. aureus, E. coli, and P. fluorescens. Methyl eugenol has been found to inhibit biofilm development by Gram-negative bacteria [77]. In previous research, efforts have been made to produce various biofilm-controlling packaging materials, especially through nanoparticle approaches such as metal oxide nanoparticles and AgNPs, silica composite materials, and biopolymer-based packaging films, to enhance their pharmacological properties and antimicrobial activity. The use of biopolymer-conjugated nanocomposites to combat the biofilm of various microorganisms is presented in Table 4.