The dose of any compound plays a key role in determining its toxicity (Podratz et al., 2011; Sadler et al., 1988; Sudmeier et al., 2015). A compound at a low dose for a shorter period to an embryo/fetus may cause a neuronal abnormality, while chronic exposure to that drug may cause lethality. Lower doses of a toxic compound affect the nervous system and thus act as a neurotoxicant.

D. melanogaster development includes stages like an embryo, larva, pupa, and adult (Fig. 1), which help in understanding the influence of myriad compounds on several organs, including the nervous system (Rand, 2010). There are several modes via which a neurotoxicant can be introduced to different developmental stages of D. melanogaster (Fig. 2). The maternal feeding method is widely used as a common mode of exposure (Fig. 2A). During maternal feeding, the exact dose of the compound consumed, metabolized, and absorbed determines the toxicity (Rand et al., 2010). For this method, the neurotoxicant of interest is mixed in food media or with diluted yeast paste for a long and short exposure (Pandey and Nichols, 2011). Often the neurotoxicants are injected into the embryo (Fig. 2B) or adults so that they are circulated to the hemolymph of the whole organism, and their toxicity can be checked (Dzitoyeva et al., 2003). Embryos are also exposed by the permeabilization method for the exposure of the neurotoxicant (Rand et al., 2010). However, due to the presence of a vitelline membrane, sometimes, direct incubation of embryos with the compound of interest fails (Rand, 2010). Thus, the vitelline membrane of the embryo is also taken out (Sabat et al., 2015) before it is exposed to the toxicants (Fig. 2C). The larvae are also injected into the neurotoxicant to study the effect (Fig. 2D). Larvae are preferred for the feeding experiments because they are a rapacious eater. Neurotoxicants like ethanol and cocaine, which are used in the form of vapor (McClung and Hirsh, 1998; Moore et al., 1998), are soaked in filter paper in the saturated form (Nichols et al., 2012) to expose the flies or larvae (Fig. 2E). Often drugs are exposed to the nerve cord of decapitated flies to see the effect (Torres and Horowitz, 1998). A number of times the drugs in introduced via glass microcapillary. This method allows the precise measurement of the drugs consumed by a single or group of flies (Ja et al., 2007). This method is popularly known as the capillary feeder assay.

Besides mode, the developmental stage in which the compound is administered also plays a central role in determining its toxicity. Thus, the same compound administered at different developmental stages shows a different result. Why do different stages respond differently to the same compound? In the embryonic stage, the fate of various cells gets determined and leads to the formation of the organ, including neurons and pathfinding for axons (Pandey and Nichols, 2011). The development of central, peripheral, motor, and interneurons occurs in the embryonic stage (Bossing et al., 1996; Brewster and Bodmer, 1996; Schmid et al., 1999). This allows a fly embryo to be used in numerous neurotoxicology studies (Rand et al., 2010). In the larval stage, the nervous system originates from the embryonic stage and differentiates as the ventral neurectoderm. The larva has fully functional circuits with conventional synapse like a neuromuscular junction, enabling it to perform motor and sensory functions. Using the nervous system, the larvae can detect food, chemical, temperature, light, and sound, as reviewed by Mishra and Barik (Mishra and Barik, 2018). The larva possesses many undifferentiated precursor organs known as imaginal discs, which later transform into adult structures during the pupa stage (Pandey and Nichols, 2011). The pupal stage is important in a developing D. melanogaster, as neuronal remodeling and new patterns of synaptogenesis occur in this period (Rand et al., 2010), which subsequently form a healthy adult nervous system capable of performing normal functions. If a chemical interferes with any of the above-mentioned developmental stages, it alters the structure and functioning of several organs, including sensory organs (Bournias-Vardiabasis and Teplitz, 1982; Bournias-Vardiabasis et al., 1983; Mellerick and Liu, 2004).

The developmental cycle of D. melanogaster is used widely to check the toxic effect of a neurotoxicant or teratogen (Bianchini et al., 2018; Li and Bi, 2018). Many developmental time points like the hatching of eggs to larva, a transition of larvae to pupae, pupation time, hatching of pupae to eclosion of adult flies, and their survivability or life span are already known (Fig. 1). Developmental time points are noted after the exposure of neurotoxicants and compared with the control flies. Alteration in the time point of any of these stages is considered a defect caused due to neurotoxicants (Bianchini et al., 2018). Besides time, the number of animals affected at the developmental stages due to the effect of a neurotoxicant is also calculated (Figs. 3A–3E). The hatching of fewer larvae suggests the death of the embryos (Fig. 3C). The dead embryos can be imaged under light and scanning electron microscope to identify the phenotypic defect (Bianchini et al., 2018; Rand et al., 2014). Similarly, if the death occurs during the larval stage, then the dead larvae (Fig. 3D) can be checked under light and scanning electron microscope to identify the phenotypic defect (Affleck et al., 2006b; Rand et al., 2014). Death may also occur in the pupa stage (Fig. 3E). The dead pupa appears black, indicating incomplete pupation (Fig. 3E), and in such cases, fewer flies are hatched (Rand et al., 2014). The dead pupa is dissected and compared with the control pupa to check the developmental defect due to the neurotoxicant. Thus, the analysis of the number of animals in all the stages gives a clear picture of the effect of the neurotoxicant in the development of D. melanogaster (Fig. 3).

A neurotoxicant can alter the size of the various developmental stages (described in the above section). The teratogen-induced morphometric developmental defect is reported for many chemicals (Lynch et al., 1991; Schuler et al., 1982). Those strategies can be adopted to check the toxic effect of neurotoxicants. Some of the neurotoxicants even produce more than one phenotypic defect. To check the abnormality of the embryo, it is collected and imaged under both light and scanning electron microscopes. Defective regions can be assessed using Image J for any kind of morphological defect. Similarly, the larvae are collected after the treatment of neurotoxicants and imaged under a stereomicroscope. From the image, the length, breadth, diameter, and area of the larvae are measured using Image J software. Similarly, in case of any phenotypic abnormality in the adult fly, an image of that region is taken and subjected to quantification to deduce a value. An increase or decrease in the size of the larva or adult flies can be measured through its weight for quantification purposes.

A teratogen can alter the cell cycle and cell division. To check the effect of a teratogen on the cell division and cell cycle, apoptosis is checked in various tissues. If the teratogen is administered orally, the midgut of the larvae is analyzed for apoptosis (Priyadarsini et al., 2020). Double staining of Caspase and DAPI/Hoescht can provide information about apoptosis in various tissues. Hoescht/DAPI staining detects the nuclei shape within the tissue (Fig. 4A). Apoptotic nuclei are smaller in size in comparison to the normal nuclei. From the shape and size of the nuclei, the apoptotic condition of the cell can be determined (Fig. 4A). DAPI binds to DNA, and Caspase binds to apoptotic cells. The TUNEL (TdT-mediated dUTP-biotin nick end labeling) assay is also used to check the apoptosis in the cell (Vasudevan and Ryoo, 2016). Several studies also use acridine orange and propidium iodide to detect cell death (Sahu and Mishra, 2020b; Vasudevan and Ryoo, 2016). Imaginal discs (eye and wing) of the larvae are stained with acridine orange to detect the cell death caused by neurotoxicants (Figs. 4B and 4C). Newly eclosed flies were examined under the stereomicroscope. After detecting the defect, they are analyzed under the scanning electron microscope for visualization at a higher resolution. The defective organ of the adult stage is analyzed in a developmental time window to detect the role of cell death and cell cycle in the formation of a phenotypic defect (Mishra et al., 2010; Sahu and Mishra, 2020a). To check the internal defect, the defective tissue of organs is fixed for histological analyses. The sections are cut, stained, and imaged under the microscope for phenotypic analysis. For the analysis of fine structural defects, the tissues are fixed and analyzed under a transmission electron microscope. Any abnormality at the organelle level can be detected after the analysis of the sample under a transmission electron microscope (Moreira et al., 2010).

Biochemical assays are used to detect the neurotoxicity of an unknown compound. Some of the common endpoints of measurements are (1) glutathione content, (2) glutathione-S-transferase, (3) lipid peroxidation, (4) protein carbonylation, (5) acetylcholinesterase (AchE) activity, (6) monoamine oxidase, and (7) caspase-9 and caspase-3 activity. Glutathione content after the treatment of neurotoxicants can be studied using Ellman’s Reagent (Jollow et al., 1974). Depletion of glutathione results in the death of the neurons (White and Cappai, 2003) because it is an essential neuronal antioxidant necessary to detoxify free radicals and prevent oxidative stress (Bains and Shaw, 1997). Glutathione-S-transferase (GST) is a major detoxifying defense enzyme of the antioxidant enzyme system (Dasari et al., 2018). GSTs activity is measured at 340 nm from the end product of the reaction. The lipid peroxidation assay measures the level of stress within the body. Stress can be estimated following the protocol of Mishra and Acharya (Mishra and Acharya, 2004) by quantifying the end product of the reaction, i.e., thiobarbituric acid (TBARs) at 535 nm. More TBARs content leads to apoptosis-induced neuronal cell death. The protein carbonyl content can be estimated following Hawkins et al. (2009). Carbonylation alters the protein functions and leads to several intermolecular aggregates and crosslinks that degrade the intracellular proteases. Thus, the accumulation of carbonylated protein content leads to several CNS disorders. Acetylcholinesterase is an essential neurotransmitter. The activity of AchE directly defines the effects of neurotoxicants on the nervous system. AchE activity can be estimated at 412 nm following Ellman and Courtney (1961). Monoamine oxidase (MAO) plays a significant role in the bioactivation of neurotoxic analogs (Heikkila et al., 1988). Although the MAO homolog is not present in the fly (Roelofs and van Haastert, 2001), its activity has been reported by several authors (Chaudhuri et al., 2007, Wang et al., 2011). Interestingly, drugs which can inhibit the activity of MAO can also inhibit the activity of flies (Yellman et al., 1997). Recently, the activity of MAO was measured from the fly head (Oyeniran et al., 2021). Lemons can protect from Alzheihmer’s disease by decreasing the MAO activity (Oyeleye et al., 2021). Capsaicin can also reduce the MAO activity in the Parkinson’s model of D. melanogaster (Siddique et al., 2018). MAO can be quantified following Mcewen (McEwen, 1965). Caspase-9 (Dronc) and Caspase-3 (Drice) reveal the neurotoxicity leading to neuronal cell death and are quantified by detecting the chromophore p-nitroanilide at 405 nm (Shakya and Siddique, 2018).

Behavioral assays are well studied to check the effect of a neurotoxicant or teratogen. A neurotoxicant/teratogen can modify the behavior of an organism by altering the expression of genes and neurotransmitters associated with it (Barik and Mishra, 2019; Moore et al., 1998; Nichols et al., 2012). The alteration of the nervous system in developmental time offers D. melanogaster as a model to study behaviors associated with the nervous system (Dhar et al., 2020b; Rand, 2010). The exposure of the embryonic stage to a neurotoxicant can interrupt the development of the nervous system and the glia associated with it (Rand et al., 2010). Those embryos later have a defective nervous system. (Bianchini et al., 2018; Rand et al., 2010). A functional nervous system makes the fly respond toward light (Hardie, 2012), odors (Montell, 2009), sound, tastants (Montell, 2009; Weiss et al., 2011), humidity (Liu et al., 2007), temperature (McKemy, 2007), and gravity (Inagaki et al., 2010; Kamikouchi et al., 2009). These responses are used to check the functionality of the nervous system by doing numerous behavioral assays in adult flies (Fig. 5). The assay is choice to detect the response towards the light (Lilly and Carlson, 1990), vision (Gerber et al., 2004), smell (Shaver et al., 1998), heat (Liu et al., 2003), and taste (Heimbeck et al., 1999). Third instar larvae are used to check for foraging behavior (Pereira et al., 1995; Sokolowski et al., 1997), light sensing ability (Busto et al., 1999), and the coordination of neuromuscular junction (Fig. 6A). The larval response towards light and determines the functionality of circadian rhythm (Luna et al., 2013). Larvae can detect the right type of food using olfaction (Kim et al., 2015), this behaviour is called feeding behavior (Fig. 7B). For this assay, yeast paste is kept at the center of the Petri plate, and the time taken by the larvae to reach the area is calculated (Min and Condron, 2005). The time taken by the control and the treatment larva is calculated and compared for the abnormality. Self-righting behavior is associated with the functioning of mechanosensory organs. The self-right test is used to keep the animal maintained at its right position. For this, the first instar larvae are placed towards the ventral side up (Fig. 6C). The time taken by the larva to turn to its original position is monitored. This behavior was recently reviewed by Dhar et al. (2020b), who used third instar larva to detect their ability to differentiate between heat and cold (Fig. 6D). To respond to the cold temperature, the larval body contracts (Turner et al., 2016) by activating class III (CIII) multicentric neurons (Turner et al., 2016). The touch-sensitive assay is used to detect the larval response towards touch by gently touching the thoracic segment (Fig. 6E) (Caldwell et al., 2003). Similarly, the larval body has numerous channels to detect the sound. To detect the sound sensing ability of the larva, sound avoidance behavioral assay is used in the third instar larvae (Dhar et al., 2020a, 2020b). If there is any defect in the channel, the larvae do not respond to the sound properly. Late third instar larvae can climb the wall of the vial to form pupae (Fig. 6F). The height climbed by the untreated and treated larvae is calculated. Larvae with defective motor neurons are unable to climb high either due to defects in the morphology or in the signaling molecule (Bianchini et al., 2018; Lozinsky et al., 2012; Lozinsky et al., 2013). Thus a defect in the sensory organ can be screened. Larvae also choose the right place for pupation for their survival. This performance is called pupation site preference (PSP) behavior (Sameoto and Miller, 1968). A larva having a defective nervous system shows altered PSP behavior in response to epileptic drugs (Beltramí et al., 2012). Temporal evaluation is also considered one of the parameters to measure behavioral teratogenesis. For this evaluation, flies are released into food-containing vials and allowed to lay eggs. After 24 h, the number of larvae are counted. Next, the formation of second and third instar larvae was checked with time. The time taken to form the first pupa and the number of pupae are counted. Similarly, the hatching time of the first fly and the number of flies hatched are counted after metamorphosis (Bianchini et al., 2018). Adult flies hatched after neurotoxic compound treatment are checked for their climbing ability against gravity (Fig. 6D). Dopamine, octopamine, tyramine, and serotonin are associated with locomotory behavior (Pendleton et al., 2000; Sombati and Hoyle, 1984; Saraswati et al., 2004; Silva et al., 2014). Several functions of the antennae, including balancing, have been reviewed by Bokolia and Mishra (Bokolia and Mishra, 2015). A defective antennae make the fly positively geotaxis. Thus, the climbing assay quantifies the neuronal defects and aging in D. melanogaster (symptoms of Parkinson’s disease) (Feany and Bender, 2000; Shaltiel-Karyo et al., 2010). Similarly, the aversive phototaxic suppression (APS) assay (Le Bourg and Buecher, 2002; Seugnet et al., 2009) is used to study Alzheimer’s, Parkinson’s, and Huntington’s diseases in the D. melanogaster model to enumerate the deviations in locomotion, learning, and memory (Ali et al., 2011). Dopamine is involved in memory formation (Berry et al., 2012) and octopamine is involved in both learning and memory. Serotonin is responsible for long-term memory (Sitaraman et al., 2015; Scheunemann et al., 2018), and gamma-aminobutyric acid (GABA) convert sleep into memory consolidation (Haynes et al., 2015). Adult flies also respond towards starvation and desiccation (Hoffmann and Harshman, 1999). Any anomaly after neurotoxicant treatment can amend this behavior, pointing to a defect in the nervous system. The larval photosensitive pattern is analyzed under a regulated light-dark cycle, and the locomotion is monitored. A neurotoxicant may alter the 24-h light–dark cycle by altering the sleep cycle (Hirsch et al., 2012). D. melanogaster Activity Monitors (DAMs) is widely used to track the locomotor activity of adult flies. Dopamine is involved in sleep and arousal (van Swinderen and Andretic, 2011), acetylcholine regulate sleep promotion (Aso et al., 2014), and glutamate (Guo et al., 2016) and serotonin (Liu et al., 2019) regulate the sleep.

Some of the D. melanogaster behaviors need the signal from many sensory organs, as recently reviewed by (Sahu et al., 2020). They are courtship, grooming, aggression, social avoidance, and predator fear behavior (Fig. 7). These behaviors depend on the signal from the eye, hearing and the chemosensory organ (Nichols et al., 2012). Many neurotransmitters are also involved in this process. Male courtship behavior is associated with dopamine and tyramine (Huang et al., 2016). Octopamine regulates both male and female courtship behavior (Zhou et al., 2012; Rezával et al., 2014). Courtship behavior follows particular steps before mating (Spieth, 1974); this behavior is used to check the functionality of neurons (Nichols et al., 2012). Any anomaly in the mating step indicates defective neurons. Defective courtship behavior is seen as a fly model of Parkinson’s disease (Shaltiel-Karyo et al., 2012). Similarly, grooming behavior helps the fly to keep itself clean. A defective mechanosensory organ resulted in faulty grooming behavior keeping the fly dirty (Sahu and Mishra, 2020a). Aggressive behavior helps the animal to find its food and partner, and protect the territory (Dhar et al., 2020a; Sahu et al., 2020). Aggressive behavior is associated with the environment, and (Dhar et al., 2020a) the level of serotonin and dopamine also regulates aggression (Alekseyenko et al., 2013). A defective aggressive behavior is associated with serotonin and the dysfunction of the antennae (Dhar et al., 2020a). When exhibiting social avoidance behavior, flies move away from a stress signal released by the stressed fly. The stress signal released by the flies is known as dS (Suh et al., 2004). For this assay, flies are placed in a T-maje device. Flies have a chance to avoid dSO present in the vial and a fresh vial. This experiment is carried out at 25°C–30°C, with even light and more than 30% humidity. After one minute of introduction to the T-maje device, flies present in both the chambers are counted. This experiment is repeated for three independent data sets and a T-test is carried out to compare the result (Fernandez et al., 2014). D. melanogaster perform different behavior in presence of a predator (Parigi et al., 2019). The behaviors include (1) lifting of the abdomen, (2) flying (moving in space using wings), (3) jumping (instantaneous movement between points without the use of the wing), (4) pausing (inactive period, similar to stopping; the duration lasts for less than one second, (5) turn orient 180° without changing the position, (6) wing display (lifting of wing without singing or vibration), (7) grooming (rolling of legs over different parts of the body while in a stationary stage), (8) walking (movement through space), (9) running (rapid movements of the body), (10) stopping (immobile for more than one second), and (11) retreating (walking in reverse direction while encountred with a predator). The predatory response behavior varies with species and different stages of development.

D. melanogaster model is used to study ethanol-induced developmental and behavioral defects. Prenatal exposure to ethanol causes reduced viability, delay in developmental time, and smaller size of the hatched flies due to less cell division (McClure et al., 2011). Ethanol vapor affects the behavior of D. melanogaster and makes the fly hyperactive, disoriented, and uncoordinated (Cohan and Hoffmann, 1986). Ethanol can target receptors and channels like voltage-gated channels, NMDA, serotonin, and GABA (Diamond and Gordon, 1997). Some of the behavioral defects in D. melanogaster are dependent on dopamine (Bainton et al., 2000). Among signaling pathways, the insulin pathway is largely affected (reduced insulin receptor and D. melanogaster insulin-like peptide (DILP)) due to the action of mediating developmental and behavioral defects (McClure et al., 2011). DILP regulates the growth, reproduction, longevity, metabolism of carbohydrates and fat (Géminard et al., 2009).

MTX, a commonly used drug in chemotherapy, is also checked for its teratogenic effect using D. melanogaster (Affleck and Walker, 2008). MTX inhibits the dihydrofolate reductase in D. melanogaster (Affleck et al., 2006a) and in humans, affects the folate metabolism, which is mediated by dihydrofolate reductase (Affleck and Walker, 2008).

MTX can cause irreversible defects in gestation and embryogenesis (Affleck et al., 2006a) in D. melanogaster, and malformed eye (photoreceptor organ), wing, bristle (mechanoreceptor organ), and the curvature of appendage are some of the developmental defects caused due to MTX (Affleck et al., 2006a).

Exposure of VOCs to flies causes defective coordination during rapid mobility, restlessness, and frequent fall during jumping near the vial. Later, the flies become sluggish in movement or suffer from bradykinesia after the exposure to the toxicant for 12–18 h. This behavior shows similarity with the fly model for Parkinson’s disease when dopamine gets affected (Inamdar et al., 2010). Low concentrations of fungal VOCs in adult D. melanogaster results in the locomotory defect by altering the activity of dopaminergic neurons (Inamdar et al., 2010). Industrial VOC exposure increases the production of reactive oxygen species (Bayil et al., 2008; Singh et al., 2009) which can induce lipid peroxidation with the production of toxic products (Inamdar et al., 2010).

Exposure to AED during the first trimester causes anatomical abnormalities, whereas exposure during the third trimester causes behavioral anomalies in D. melanogaster. AEDs such as phenytoin, valproic acid (VPA), and carbamazepine alter the genotoxicity of D. melanogaster in a dose-dependent manner (Yüksel et al., 2010). Mating propensity, larval PSP, and climbing ability are largely affected after treatment with AEDs (Harini, 2016). AEDs are reported to act via the GABA channel, which along with dopamine, regulates the female receptivity during courtship and mating (Gayathri and Harini, 2012). VPA reduces the copulation duration, whereas pentylenetetrazole amends the climbing speed of adult flies. The larva changes the PSP concerning different AEDs. PSP has an important role in pre-adult development as per the survivability of pupae (hence adult D.melanogaster) is concerned (Harini, 2016). A high dose of phenytoin resulted in death in all the developmental stages of D. melanogaster (Gayathri and Harini, 2013).

It is a potent psychostimulant in D. melanogaster. A low dose of cocaine can make flies hyperactive, uncontrollable, and continuous grooming behavior as a defective behavioral endpoint. A locomotory defect such as circling behavior is seen among flies (Schafer, 2002). A high dose of cocaine was shown to induce irregular activity, shock, and complete immobility (akinesia) in D. melanogaster (Bainton et al., 2000). Repeated exposure of flies to cocaine increases the behavioral response (sensitization) (Schafer, 2002) and male flies show more sensitivity towards cocaine than females. Alteration of dopamine levels can change the grooming and locomotive behaviors of D. melanogaster (Torres and Horowitz, 1998).

The effect of nicotine is confined to the CNS in D. melanogaster since acetylcholine receptors for nicotine in insects are found only in the nervous system (Gundelfinger, 1992; Restifo and White, 1990). Nicotine elicits similar behavioral defects as cocaine. Volatilized nicotine exposure can make flies hyperactive and induce occasional movements, resulting in grooming, hypokinesis, and akinesia in the maximum treated individuals, along with impaired negative geotaxis movement (Bainton et al., 2000; Heberlein et al., 2009). Interestingly, flies treated with both cocaine and nicotine simultaneously show impaired climbing ability in a dose-dependent manner (Bainton et al., 2000).

Oral ingestion of hydrogen peroxide (0.1%–2%) as well as the injection of hydrogen peroxide (1%) can increase the locomotory activity of adult flies. Injected hydrogen peroxide mixed with the hemolymph thus causes an alteration of the activity of the fly. Superoxide dismutase (SOD), a hydrogen peroxide-producing enzyme, also increases fly activity. Injection of hydrogen peroxide and expression of SOD resulted in abnormal walking movement in flies (Grover et al., 2009).

Environmental stress, such as thermal stress, can disrupt the CNS (Wang et al., 2007). The mushroom body (MB) is associated with the integration of sensory and associated center of the insect brain, which is further responsible for memory and conditioned behaviors (Davis, 2005). Thermal exposure of larva and pupa during development can change the MB by reducing intrinsic Kenyon cells and the number of neurons by affecting the associative odor learning in adults (Bahrndorff et al., 2016; Wang et al., 2007). Similarly, brief exposure to a weak static magnetic field during the early period of development greatly affects the cuticular pattern of D. melanogaster larva (Ho et al., 1992) and the negative geotaxis behavior of adults (Fedele et al., 2014). The hatching rate of the larva decreases due to weak static magnetic fields (Ramirez et al., 1983).

Elements are essential for several biological activities in an organism, and their roles are conserved between D. melanogaster and higher vertebrates (Calap-Quintana et al., 2017). The human body utilizes metals from food. The body adjusts to the deficiency or excess of metals at the cellular level by altering the metabolic pathways. In the fly, the metals are metabolised using the secretory pathways. If the concentration of the metal exceeds the tolerance level within the body for any reason, it causes neurotoxicity or teratogenicity in flies. We have listed a few elements which are validated as teratogen using D. melanogaster.

Hg is an environmental pollutant and teratogen in D. melanogaster. Hg increases the larva to pupa transition time, and reduces the size of the larva, pupae, and hatching rate of pupae. It affects the phosphorylation of various kinases such as MAPK, extracellular signal-regulated kinase (ERK), and c-JUN N-terminal kinase (JNK). In D. melanogaster, ERK regulates cell growth and differentiation (Posser et al., 2009), whereas JNK is responsible for cell cytoskeleton and cell formation (Pereira et al., 2011). The toxic effects of Hg include non-functioning of the nervous system and glia (Rand et al., 2009), polarity loss in follicular cells (Baffet et al., 2009), and differentiation defect of nerve and muscle cell (Bournias-Vardiabasis et al., 1990). Abnormal cell differentiation during metamorphosis was also observed in the latter study. More importantly, Hg inhibits the notch pathway and thus disrupts normal neural development (Engel et al., 2012). The inhibition of the notch pathway reduces the hatching rate of the larva (Abnoos et al., 2013).

Pb²⁺ can change the developmental neuronal plasticity in D. melanogaster (Jin et al., 2005). Ca²⁺ contributes to synaptic development at different steps, such as guiding the growth cone (Jin et al., 2005), the formation of the synapse (Xu et al., 2009), elimination, and stabilization (Lohmann and Bonhoeffer, 2008; Pratt et al., 2003). Pb²⁺ affects Ca²⁺ binding proteins (Hirsch et al., 2012) and causes abnormal larval neuromuscular junction and mitochondria with defective ATP synthesis (Flora et al., 2008). Less ATP delays pupal development (Hirsch et al., 2012). Recently it was found that behavioral abnormality induced by Pb²⁺ in flies is by altering the microbiota (Sun et al., 2020).

Chromium exists in various forms like Cr(III)Cl₃, K₂Cr(VI)O_4, and K₂Cr(VI)₂O_7, and its toxicity was checked using D. melanogaster. Wing spots are seen in the adults when the larvae were fed with Cr (VI) salt (Katz et al., 2001; Yeşilada, 2001). Feeding CrCl₃ does not cause any wing spots in adults. [Cr(pic)₃], another chromium (III) compound was fed at a concentration of 200–600 μg per day (Hepburn et al., 2003). In the offspring, it causes a delay in development and death during the process of development (Hepburn et al., 2003). [Cr(pic)₃] does not cause any phenotypic or behavioral, or survival defect when fed to the adult flies. [Cr(pic)₃] does not cause defects genetically but interferes with the metabolic pathways (Hepburn et al., 2003).

Ni exposure is known to exert many epidemiological and mutagenic effects on human beings. The Ni effect was investigated using D. melanogaster. Two different Ni compounds (NiCl₂ and NiSO₄) were employed to check the toxic effect of Ni (Carmona et al., 2011). The genotoxic potential of two Ni-compounds was assessed using D. melanogaster using the wing-spot assay and comet assay in the hemolymph. Single- and double-strand DNA breaks were detected after Ni exposure. The frequencies of the wing spot do not increase significantly. However, NiSO₄ can significantly induce DNA damage, as evidenced by comet assay (Carmona et al., 2011).

Wild type (Canton S) flies were grown on Al mixed food media (20–240 mg/kg). Larvae grown in Al-supplemented food have defective walking behavior. The locomotory activity decreases with the increasing concentration of alumina. The number of pupae and imago count decreases after the treatment of alumina (Kijak et al., 2014). The adult flies hatched after alumina treatment have defective sleep cycle and arrhythmic behavior. The toxicity of alumina depends on concentration and time of exposure. Alumina further decreases the life span of the flies. At 120 mg/kg, the male life span, as well as locomotor activity, were found to be increased (Kijak et al., 2014).

Dietary intake of several concentrations of Zn (0.1 to 1000 ppm) was checked by mixing it with the fly food (Al-Momani and Massadeh, 2005). Third instar larvae grown on Zn-added food did not exhibit altered growth and development in the first generation up to 500 ppm. At 500 ppm, 75% of pupa and adult death occurred. At 1000 ppm, survival percentages were further significantly reduced (35%) for pupa and adult stages. For the second generation, survival, growth, and development remain unaltered up to 100 ppm; however, at 500 ppm, a significant reduction in these activities was seen (Al-Momani and Massadeh, 2005). Zn upregulates the expression of metallothionein, and thus serves as an antioxidant. Zn can prevent apoptosis and thus prevent oxidative damage in the brain (Kocatürk et al., 1996). Oral supplementation of ZnCl₂ at a concentration of 4 mM to parkin mutant flies (park^25/25) increased the eclosion frequency and life span of the adult (Saini and Schaffner, 2010). The same concentration of ZnCl₂ decreases the life span of the adults and alters the feeding behavior.

Cu has a role in antioxidant mechanisms, the formation of pigments, and neurotransmitters. It is used in many fungicides. The teratogenic effect of Cu was checked using D. melanogaster. Several concentrations (40 to 320 mgL⁻¹) of CuSO₄ were mixed with the fly food (Ding and Wang, 2006) and fed to the first instar larvae. At low concentration, the larval growth rate was slow; with the increasing concentration of Cu, the larval body length decreased (Ding and Wang, 2006). At 320 mgL⁻¹ concentration, the larval body length decreased, and they took 11 days to reach the pupae stage compared to 5 days for control flies (Ding and Wang, 2006).

CoCl₂ was administered orally and the adults hatched from the pupae were analyzed for morphological defects. Trans-heterozygous larvae for the multiple wing hairs (mwh) and flare (flr) were used in this study. The wing spots were observed in the wing after treatment with CoCl₂. CoCl₂ can induce both small and large spots in the wings (Ogawa et al., 1994). In mwh/TM3 flies, CoCl₂ could not induce large spots in the wings due to suppressed mitotic crossing-over. To induce large spots, mwh/flr system clones were generated.

Mn is an essential element but can induce toxicity if it exceeds the limit. Several concentrations of MnCl₂ were supplemented to the food medium (at 0.1, 0.5, or 1 mM)(Ternes et al., 2014). MnCl₂ treatment was given throughout the development from the egg to the adult stage. Significantly enhanced locomotor activity was observed at 0.5 and 1 mM of Mn. These concentrations can induce reactive oxygen species within the body. At 1 mM, Mn can increase the mRNA expression level of catalase, superoxide dismutase, and Hsp83 without altering the activity. Mn can increase the activity of thioredoxin reductase and GST (Ternes et al., 2014).

Li affects the phosphoinositide signaling pathway and thus distresses neuronal function (Schafer, 2002). Alteration of the inositol polyphosphate pool can cause defects in synaptic function and plasticity (Acharya et al., 1998; de Camilli et al., 1996). Li₂CO₃ is a well-known sedative in psychiatric literature. Li has a role in aging, and at a moderate dose, it has a beneficial effect on longevity. Li treatment affects male mating success and female fecundity (Matsagas et al., 2009). However, chronic treatment with a low to moderate dose of lithium chloride does not alter the lifespan of D. melanogaster (Zhu et al., 2015). Affymetrix Genome Arrays from the Li-treated head of mRNA of D. melanogaster suggest an alteration of 12 genes associated with amino acid metabolism and functioning of the nervous system (Kasuya et al., 2009).